Controlling the Major Factors Impacting Trypsin Digestion
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چکیده
It is rarely the case that an analyst is presented with a sample in a suitable form for injection into an LC/MS system. Often the sample must be manipulated to concentrate the compound of interest, remove interferences, enzymatically digest the sample to generate surrogate peptides with better mass spectrometric properties, and modify the sample matrix or buffering system to achieve compatibility with LC separation and mass spectrometric detection systems.
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